Institut de Genetique et Microbiologie

 G P M S
Genomes, Polymorphism and Minisatellites


Identification of tandem repeats :


Although tandem repeats have been identified in the past by screening of genomic libraries (cosmids for instance), the availability of whole-genome sequence data has opened the way to the direct identification of tandem repeats by sequence analysis. The laboratory has developed a database for this purpose, which can be accessed at http://minisatellites.u-psud.fr (Vergnaud and Denoeud, Genome Research july 2000). In particular, the use of this tool was demonstrated in "A tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis" . The current status of this ressource has been described in more details in Denoeud F, Vergnaud G. (Identification of polymorphic tandem repeats by direct comparison of genome sequence from different bacterial strains : a web-based resource.
BMC Bioinformatics. 2004 Jan 12;5(1):4.).

 

  

 

 

 

 

 



Institut de Genetique et Microbiologie

 G P M S
Genomes, Polymorphism and Minisatellites


Strain identification of pathogenic bacteria :

The use of tandem repeats for bacterial strain identification has been illustrated and discussed in (Le Flèche et al. 2001, 2002), and a service accessible via the internet has been developed and can be accessed at http://bacterial-genotyping.igmors.u-psud.fr/.  

 

 

 



Institut de Genetique et Microbiologie

 G P M S
Genomes, Polymorphism and Minisatellites


Microbiological analysis of air samples :


The microbiological composition and fluctuations of ambiant air have not been studied in much details. The laboratory has undertaken a small pilot project in which bacteria from air samples are characterised at the genus level by 16S typing.

 

 



Institut de Genetique et Microbiologie

 G P M S
Genomes, Polymorphism and Minisatellites


Publications : 

Pourcel C, Salvignol G, Vergnaud G.
CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies.
Microbiology. 2005 Mar;151(Pt 3):653-63
Ramisse V, Houssu P, Hernandez E, Denoeud F, Hilaire V, Lisanti O, Ramisse F, Cavallo JD, Vergnaud G.
Variable number of tandem repeats in Salmonella enterica subsp. enterica for typing purposes.
J Clin Microbiol. 2004 Dec;42(12):5722-30.
Lespinasse J, Testard H, Nugues F, Till M, Cordier MP, Althuser M, Amblard F, Fert-Ferrer S, Durand C, Dalmon F, Pourcel C, Jouk PS.
A submicroscopic unbalanced subtelomeric translocation t(2p;10q) identified by fluorescence in situ hybridization: fetus with increased nuchal translucency and normal standard karyotype with later growth and developmental delay, rhombencephalosynapsis (RES).
Ann Genet. 2004 Oct-Dec;47(4):405-17.
Fabre M, Koeck JL, Le Fleche P, Simon F, Herve V, Vergnaud G, Pourcel C.
High genetic diversity revealed by variable-number tandem repeat genotyping and analysis of hsp65 gene polymorphism in a large collection of "Mycobacterium canettii" strains indicates that the M. tuberculosis complex is a recently emerged clone of "M. canettii".
J Clin Microbiol. 2004 Jul;42(7):3248-55.
Pourcel C, Andre-Mazeaud F, Neubauer H, Ramisse F, Vergnaud G.
Tandem repeats analysis for the high resolution phylogenetic analysis of Yersinia pestis.
BMC Microbiol. 2004 Jun 08;4(1):22.

Denoeud F, Vergnaud G.

Identification of polymorphic tandem repeats by direct comparison of genome sequence from different bacterial strains : a web-based resource.
BMC Bioinformatics. 2004 Jan 12;5(1):4.

Onteniente L, Brisse S, Tassios PT, Vergnaud G.

Evaluation of the polymorphisms associated with tandem repeats for Pseudomonas aeruginosa strain typing.
J Clin Microbiol. 2003 Nov;41(11):4991-7.

 

Ramisse V, Balandreau J, Thibault F, Vidal D, Vergnaud G, Normand P.

DNA-DNA hybridization study of Burkholderia species using genomic DNA macro-array analysis coupled to reverse genome probing.
Int J Syst Evol Microbiol. 2003 May;53(Pt 3):739-46.

Pourcel C, Vidgop Y, Ramisse F, Vergnaud G, Tram C.

Characterization of a tandem repeat polymorphism in Legionella pneumophila and its use for genotyping.
J Clin Microbiol. 2003 May;41(5):1819-26.

Denoeud F, Vergnaud G, Benson G.

Predicting human minisatellite polymorphism.
Genome Res. 2003 May;13(5):856-67. Epub 2003 Apr 14.

Der-Sarkissian H, Vergnaud G, Borde YM, Thomas G, Londono-Vallejo JA.

Segmental polymorphisms in the proterminal regions of a subset of human chromosomes.
Genome Res. 2002 Nov;12(11):1673-8.

Le Fleche P, Fabre M, Denoeud F, Koeck JL, Vergnaud G.

High resolution, on-line identification of strains from the Mycobacterium tuberculosis complex based on tandem repeat typing.
BMC Microbiol. 2002 Nov 27;2(1):37.

Le Fleche P, Hauck Y, Onteniente L, Prieur A, Denoeud F, Ramisse V, Sylvestre P, Benson G, Ramisse F, Vergnaud G.

 Free in PMC ,  

A tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis.
BMC Microbiol. 2001;1(1):2.

 

Giraudeau F, Taine L, Biancalana V, Delobel B, Journel H, Missirian C, Lacombe D, Bonneau D, Parent P, Aubert D, Hauck Y, Croquette MF, Toutain A, Mattei MG, Loiseau HA, David A, Vergnaud G.

Use of a set of highly polymorphic minisatellite probes for the identification of cryptic 1p36.3 deletions in a large collection of patients with idiopathic mental retardation.
J Med Genet. 2001 Feb;38(2):121-5
PMID: 11288712   

 

Valenza-Schaerly P, Pickard B, Walter J, Jung M, Pourcel L, Reik W, Gauguier D, Vergnaud G, Pourcel C.

A dominant modifier of transgene methylation is mapped by qtl analysis to mouse chromosome 13.
Genome Res. 2001 Mar;11(3):382-8.
PMID: 11230162   

Livshits LA, Malyarchuk SG, Kravchenko SA, Matsuka GH, Lukyanova EM, Antipkin YG, Arabskaya LP, Petit E, Giraudeau F, Gourmelon P, Vergnaud G, Le Guen B.

Children of Chernobyl Cleanup Workers do not Show Elevated Rates of Mutations in Minisatellite Alleles.
Radiat Res. 2001 Jul;155(1):74-80.
PMID: 11121218

Vergnaud G, Denoeud F.

Minisatellites: mutability and genome architecture.
Genome Res. 2000 Jul;10(7):899-907. Review.

Vergnaud G.

Structure and evolution of human sub-telomeric regions.
J Soc Biol. 1999;193(1):35-40. Review. French.

Dai KZ, Vergnaud G, Ando A, Inoko H, Spurkland A.

The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene cluster on human Chromosome 1.
Immunogenetics. 2000 Mar;51(3):179-85.

Debrauwere H, Buard J, Tessier J, Aubert D, Vergnaud G, Nicolas A.

Meiotic instability of human minisatellite CEB1 in yeast requires DNA double-strand breaks.
Nat Genet. 1999 Nov;23(3):367-71.

Giraudeau F, Petit E, Avet-Loiseau H, Hauck Y, Vergnaud G, Amarger V.

Finding new human minisatellite sequences in the vicinity of long CA-rich sequences.
Genome Res. 1999 Jul;9(7):647-53.

 

Giraudeau F, Apiou F, Amarger V, Kaisaki PJ, Bihoreau MT, Lathrop M, Vergnaud G, Gauguier D.

Linkage and physical mapping of rat microsatellites derived from minisatellite loci.
Mamm Genome. 1999 Apr;10(4):405-9. 

Hoyheim B, Amarger V, Vergnaud G, Thomsen PD.

A polymorphic porcine dinucleotide repeat S0559 (BHT0107) at chromosome 6q28-q31.
Anim Genet. 1998 Dec;29(6):460. 

Amarger V, Gauguier D, Yerle M, Apiou F, Pinton P, Giraudeau F, Monfouilloux S, Lathrop M, Dutrillaux B, Buard J, Vergnaud G.

Analysis of distribution in the human, pig, and rat genomes points toward a general subtelomeric origin of minisatellite structures.
Genomics. 1998 Aug 15;52(1):62-71.

Monfouilloux S, Avet-Loiseau H, Amarger V, Balazs I, Pourcel C, Vergnaud G.

Recent human-specific spreading of a subtelomeric domain.
Genomics. 1998 Jul 15;51(2):165-76.

Dubrova YE, Nesterov VN, Krouchinsky NG, Ostapenko VA, Vergnaud G, Giraudeau F, Buard J, Jeffreys AJ.

Further evidence for elevated human minisatellite mutation rate in Belarus eight years after the Chernobyl accident.
Mutat Res. 1997 Nov 28;381(2):267-78.

Giraudeau F, Aubert D, Young I, Horsley S, Knight S, Kearney L, Vergnaud G, Flint J.

Molecular-cytogenetic detection of a deletion of 1p36.3.
J Med Genet. 1997 Apr;34(4):314-7.

Cox SA, Attwood J, Bryant SP, Bains R, Povey S, Rebello M, Kapsetaki M, Moschonas NK, Grzeschik KH, Otto M, Dixon M, Sudworth HE, Kooy RF, Wright A, Teague P, Terrenato L, Vergnaud G, Monfouilloux S, Weissenbach J, Alibert O, Dib C, Faure S, Bakker E, Pearson NM, Spurr NK, et al.

European Gene Mapping Project (EUROGEM): breakpoint panels for human chromosomes based on the CEPH reference families. Centre d'Etude du Polymorphisme Humain.
Ann Hum Genet. 1996 Nov;60 ( Pt 6):447-86.

Caron H, van Sluis P, Buschman R, Pereira do Tanque R, Maes P, Beks L, de Kraker J, Voute PA, Vergnaud G, Westerveld A, Slater R, Versteeg R.

Allelic loss of the short arm of chromosome 4 in neuroblastoma suggests a novel tumour suppressor gene locus.
Hum Genet. 1996 Jun;97(6):834-7.

Foucault F, Buard J, Praz F, Jaulin C, Stoppa-Lyonnet D, Vergnaud G, Amor-Gueret M.

Stability of microsatellites and minisatellites in Bloom syndrome, a human syndrome of genetic instability.
Mutat Res. 1996 Apr 2;362(3):227-36.

Pravenec M, Gauguier D, Schott JJ, Buard J, Kren V, Bila V, Szpirer C, Szpirer J, Wang JM, Huang H, St Lezin E, Spence MA, Flodman P, Printz M, Lathrop GM, Vergnaud G, Kurtz TW.

A genetic linkage map of the rat derived from recombinant inbred strains.
Mamm Genome. 1996 Feb;7(2):117-27.

Schott JJ, Charpentier F, Peltier S, Foley P, Drouin E, Bouhour JB, Donnelly P, Vergnaud G, Bachner L, Moisan JP, et al.

Mapping of a gene for long QT syndrome to chromosome 4q25-27.
Am J Hum Genet. 1995 Nov;57(5):1114-22.

Kozman HM, Keith TP, Donis-Keller H, White RL, Weissenbach J, Dean M, Vergnaud G, Kidd K, Gusella J, Royle NJ, et al.

The CEPH consortium linkage map of human chromosome 16.
Genomics. 1995 Jan 1;25(1):44-58.

Vergnaud G.

No increase in female recombination frequency in the distal part of the human pseudoautosomal region.
Genomics. 1994 Dec;24(3):610-2.

Buard J, Vergnaud G.

Complex recombination events at the hypermutable minisatellite CEB1 (D2S90).
EMBO J. 1994 Jul 1;13(13):3203-10.

Riley BP, Williamson R, Vergnaud G.

The EUROGEM map of human chromosome 22.
Eur J Hum Genet. 1994;2(3):246-7. 

Wunderle V, Dib C, Fizames C, Morisette J, Hazan J, Hansmann I, Whitehouse D, Vergnaud G, Weissenbach J.

The EUROGEM map of human chromosome 20.
Eur J Hum Genet. 1994;2(3):242-3. 

Cox SA, Harley H, Akhtar RA, Kruse T, Vergnaud G.

The EUROGEM map of human chromosome 19.
Eur J Hum Genet. 1994;2(3):240-1. 

Cox SA, Vergnaud G, Milan J, Moreno F, Beckmann J, Kruse T, Contu L.

The EUROGEM map of human chromosome 17.
Eur J Hum Genet. 1994;2(3):236-7. 

Cox SA, de Almeida S, Rosa H, Vergnaud G, Kruse T, Lavinha J.

The EUROGEM map of human chromosome 16.
Eur J Hum Genet. 1994;2(3):234-5. 

Joyce C, Fanning L, Malcolm S, Richard I, Broux O, Beckmann JS, Flint T, Kruse TA, Vergnaud G, Cox SA, et al.

The EUROGEM map of human chromosome 15.
Eur J Hum Genet. 1994;2(3):232-3. 

Attwood J, Kruse T, Vergnaud G, Malaspina P, Povey S.

The EUROGEM map of human chromosome 14.
Eur J Hum Genet. 1994;2(3):230-1

Kooy RF, Wijngaard A, Verlind E, Vergnaud G, Scheffer H, Buys CH.

The EUROGEM map of human chromosome 13.
Eur J Hum Genet. 1994;2(3):228-9. 

Bosch A, Banchs I, Puig A, Vergnaud G, Allamand V, Estivill X.

The EUROGEM map of human chromosome 12.
Eur J Hum Genet. 1994;2(3):226-7.

Povey S, Attwood J, Bakker E, Swallow DM, Vergnaud G.

The EUROGEM map of human chromosome 11.
Eur J Hum Genet. 1994;2(3):224-5. 

Kapsetaki M, Kokkinaki M, Angelicheva D, Lubyova B, Mavraki H, Argyrokastritis A, Vergnaud G, Ferguson-Smith M, Rubinsztein DC, Lush M, et al.

The EUROGEM map of human chromosome 10.
Eur J Hum Genet. 1994;2(3):222-3.

Attwood J, Vergnaud G, Lush ML, Rubinsztein DC, Goudie D, Ferguson-Smith M, Povey S.

The EUROGEM map of human chromosome 9.
Eur J Hum Genet. 1994;2(3):220-1. 

Cox SA, Spurr NK, Vergnaud G, Contu L, Carcassi C, McCarthy T.

The EUROGEM map of human chromosome 8.
Eur J Hum Genet. 1994;2(3):218-9. 

Badbanchi F, Otto M, Kohlhauer U, Grzeschik N, Beck S, Smith SJ, Swallow DM, Kooy RF, Vergnaud G, Aldred M, et al.

The EUROGEM map of human chromosome 7.
Eur J Hum Genet. 1994;2(3):216-7. 

Terrenato L, Jodice C, Blasi P, Loizedda A, Contu L, Buard J, Vergnaud G, Humphries P, Kumar-Singh R, Massart C, et al.

The EUROGEM map of human chromosome 6.
Eur J Hum Genet. 1994;2(3):214-5. 

Bakker E, Vossen RH, Riley BP, Sherrington R, Vergnaud G, Pearson NM.

The EUROGEM map of human chromosome 4.
Eur J Hum Genet. 1994;2(3):210-1. 

Schurmann M, Muller B, Duvigneau C, Leutelt J, Krey S, Kumar-Singh R, Lush M, Swallow DM, Vergnaud G, Bakker E, et al.

The EUROGEM map of human chromosome 3.
Eur J Hum Genet. 1994;2(3):208-9. 

Flint TJ, Hertz JM, Vergnaud G, Orru S, Harvey CB, Bakker B, Kruse TA.

The EUROGEM map of human chromosome 2.
Eur J Hum Genet. 1994;2(3):206-7. 

Kumar-Singh R, Wang H, Carritt B, Kruse TA, McCarthy TV, Vergnaud G, Humphries P.

The EUROGEM map of human chromosome 1.
Eur J Hum Genet. 1994;2(3):204-5. 

Bowcock AM, Gerken SC, Barnes RI, Shiang R, Jabs EW, Warren AC, Antonarakis S, Retief AE, Vergnaud G, Leppert M, et al.

The CEPH consortium linkage map of human chromosome 13.
Genomics. 1993 May;16(2):486-96.

Gurrieri F, Trask BJ, van den Engh G, Krauss CM, Schinzel A, Pettenati MJ, Schindler D, Dietz-Band J, Vergnaud G, Scherer SW, et al.

Physical mapping of the holoprosencephaly critical region on chromosome 7q36.
Nat Genet. 1993 Mar;3(3):247-51.

Vergnaud G, Gauguier D, Schott JJ, Lepetit D, Lauthier V, Mariat D, Buard J.

Detection, cloning, and distribution of minisatellites in some mammalian genomes.
EXS. 1993;67:47-57.

Mariat D, De Gouyon B, Julier C, Lathrop M, Vergnaud G.

Genetic mapping through the use of synthetic tandem repeats in the mouse genome.
Mamm Genome. 1993;4(3):135-40.

Peter M, Michon J, Vielh P, Neuenschwander S, Nakamura Y, Sonsino E, Zucker JM, Vergnaud G, Thomas G, Delattre O.

PCR assay for chromosome 1p deletion in small neuroblastoma samples.
Int J Cancer. 1992 Oct 21;52(4):544-8.

Armour JA, Vergnaud G, Crosier M, Jeffreys AJ.

Isolation of human minisatellite loci detected by synthetic tandem repeat probes: direct comparison with cloned DNA fingerprinting probes.
Hum Mol Genet. 1992 Aug;1(5):319-23.

Blanche H, Wright LG, Vergnaud G, de Gouyon B, Lauthier V, Silver LM, Dausset J, Cann HM, Spielman RS.

Genetic mapping of three human homologues of murine t-complex genes localizes TCP10 to 6q27, 15 cM distal to TCP1 and PLG.
Genomics. 1992 Apr;12(4):826-8.

Lauthier V, Vergnaud G.

CEB 13 detects a VNTR locus (Het: 93%) on chromosome 7q.
Hum Mol Genet. 1992 Apr;1(1):64. 

Lauthier V, Buard J, Delattre O, Zucman J, Vergnaud G.

CEB 29 detects a VNTR locus (Het: 80%) on chromosome XY.
Hum Mol Genet. 1992 Apr;1(1):63. 

Lauthier V, Mariat D, Vergnaud G.

CEB 15 detects a VNTR locus (Het: 92%) on chromosome 1p.
Hum Mol Genet. 1992 Apr;1(1):63. 

Mariat D, Vergnaud G.

Detection of polymorphic loci in complex genomes with synthetic tandem repeats.
Genomics. 1992 Mar;12(3):454-8.

Mariat D, Guerin G, Bertaud M, Vergnaud G.

Modulation of polymorphic loci detection with synthetic tandem repeat variants.
Mamm Genome. 1992;3(10):546-9.

Plaetke R, Weber J, Wood S, Dean M, Jeffreys AJ, Weiffenbach B, Vergnaud G, Vogelstein B, White R.

Report of the second international workshop on human chromosome 5 mapping: consensus genetic map.
Cytogenet Cell Genet. 1992;61(4):237-42. 

Vergnaud G, Mariat D, Apiou F, Aurias A, Lathrop M, Lauthier V.

The use of synthetic tandem repeats to isolate new VNTR loci: cloning of a human hypermutable sequence.
Genomics. 1991 Sep;11(1):135-44.

Mariat D, Lauthier V, Vergnaud G.

A VNTR isolated by size selection of human DNA fragments detects RFLPs at the extremity of 1p and 4q.
Nucleic Acids Res. 1991 Aug 25;19(16):4572.

Lauthier V, Mariat D, Zoroastro M, Vergnaud G.

A synthetic probe STR 14C19, detects a new polymorphic locus at 16pter (D16S282).
Nucleic Acids Res. 1991 Jul 25;19(14):4015. 

Lauthier V, Mariat D, Zoroastro M, Vergnaud G.

A synthetic probe, STR 16C17, detects a new polymorphic locus at 17pter (D17S450).
Nucleic Acids Res. 1991 Jul 25;19(14):4014. 

Lauthier V, Mariat D, Zoroastro M, Vergnaud G.

A synthetic probe, STR 14C13, detects a new polymorphic locus on chromosome arm 7q (D7S450).
Nucleic Acids Res. 1991 Jul 25;19(14):4014.

Lauthier V, Mariat D, Zoroastro M, Vergnaud G.

A synthetic probe, STR 16C2, detects a new polymorphic locus at 5pter (D5S206).
Nucleic Acids Res. 1991 Jul 25;19(14):4013.

Lauthier V, Mariat D, Zoroastro M, Vergnaud G.

A synthetic probe, STR 16C18, detects a new polymorphic locus at 12qter (D12S55).
Nucleic Acids Res. 1991 Jul 25;19(14):4013.

Vergnaud G, Mariat D, Zoroastro M, Lauthier V.

Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides.
Electrophoresis. 1991 Feb-Mar;12(2-3):134-40.

Vergnaud G.

Polymers of random short oligonucleotides detect polymorphic loci in the human genome.
Nucleic Acids Res. 1989 Oct 11;17(19):7623-30.

 



Institut de Genetique et Microbiologie

 G P M S
Genomes, Polymorphism and Minisatellites


Laboratory composition :

     Short term student reports (in french)

     Current laboratory members : 

                        e-mail suffix: @igmors.u-psud.fr

Olivier Gorgé (pharmacien)
GPMS, Bat 400
Institut de Génétique et Microbiologie
Université Paris-Sud
91405 Orsay Cedex (France)
tel : +33 (0) 1 69 15 46 45
fax : +33 (0) 1 69 15 66 78
contact: Olivier.Gorge + suffix

Yolande Hauck (Assistante ingénieur)
GPMS, Bat 400
Institut de Génétique et Microbiologie
Université Paris-Sud
91405 Orsay Cedex (France)
tel : +33 (0) 1 69 15 46 45
fax : +33 (0) 1 69 15 66 78
contact: Yolande.Hauck + suffix

Philippe Le Flèche (Ingénieur Etudes et Fabrication)
GPMS, Bat 400
Institut de Génétique et Microbiologie
Université Paris-Sud
91405 Orsay Cedex (France)
tel : +33 (0) 1 69 15 46 45
fax : +33 (0) 1 69 15 66 78
contact: lefleche + suffix

Zeina Majed (PhD student)
GPMS
, Bat 400
Institut de Génétique et Microbiologie
Université Paris-Sud
91405 Orsay Cedex (France)
tel : +33 (0) 1 69 15 70 05
fax : +33 (0) 1 69 15 66 78
contact : Zeina.Majed + suffix

Christine Pourcel (on leave, Institut Pasteur, 2002-2003)
GPMS, Bat 400
Institut de Génétique et Microbiologie
Université Paris-Sud
91405 Orsay Cedex (France)
tel : +33 (0) 1 69 15 30 01
fax : +33 (0) 1 69 15 66 78
contact : Christine.Pourcel + suffix

Samina Valjevac (diplôme ingénieur, since 2002)
GPMS, Bat 400
Institut de Génétique et Microbiologie
Université Paris-Sud
91405 Orsay Cedex (France)
tel : +33 (0) 1 69 15 70 05
fax : +33 (0) 1 69 15 70 05
contact  : Samina.Valjevac + suffix

Gilles Vergnaud (Ing. Chef Arm.)
GPMS, Bat 400
Institut de Génétique et Microbiologie
Université Paris-Sud
91405 Orsay Cedex (France)
tel : +33 (0) 1 69 15 62 08
fax : +33 (0) 1 69 15 66 78
e-contact : Gilles.Vergnaud + suffix